EliKine Human beta-NGF ELISA Kit

Artikelnummer: BYT-ORB3145543
Artikelname: EliKine Human beta-NGF ELISA Kit
Artikelnummer: BYT-ORB3145543
Hersteller Artikelnummer: orb3145543
Alternativnummer: BYT-ORB3145543-48, BYT-ORB3145543-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: Beta nerve growth factor, Beta NGF, HSAN5, MGC161426, MGC161428, Nerve growth factor (beta polypeptide), Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NGFB, NID67
EliKine Human beta-NGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human beta-NGF.
Detektionsbereich: 31.25 pg/mL- 2000 pg/mL
Sensitivitaet: 16 pg/mL
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: EliKine Human beta-NGF ELISA Kit employs a two-site sandwich ELISA to quantitate beta-NGF in samples. An antibody specific for beta-NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-NGF is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-NGF bound in the initial step. The color development is stopped and the intensity of the color is measured