EliKine Human beta-NGF ELISA Kit

Product Overview

• Article Name: EliKine Human beta-NGF ELISA Kit
• Biozol Catalog Number: BYT-ORB3145543
• Supplier Catalog Number: orb3145543
• Alternative Catalog Number: BYT-ORB3145543-48, BYT-ORB3145543-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: Beta nerve growth factor, Beta NGF, HSAN5, MGC161426, MGC161428, Nerve growth factor (beta polypeptide), Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NGFB, NID67

Product Description

EliKine Human beta-NGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human beta-NGF.

Product Properties

• Range: 31.25 pg/mL- 2000 pg/mL
• Sensitivity: 16 pg/mL
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: EliKine Human beta-NGF ELISA Kit employs a two-site sandwich ELISA to quantitate beta-NGF in samples. An antibody specific for beta-NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-NGF is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-NGF bound in the initial step. The color development is stopped and the intensity of the color is measured