EliKine Mouse IL-4 ELISA Kit

Artikelnummer: BYT-ORB3145556
Artikelname: EliKine Mouse IL-4 ELISA Kit
Artikelnummer: BYT-ORB3145556
Hersteller Artikelnummer: orb3145556
Alternativnummer: BYT-ORB3145556-48, BYT-ORB3145556-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Mouse
Alternative Synonym: IL 4, IL4, BCGF-1, BCGF1, BSF1, IL-4, MGC79402, B cell growth factor 1, B_cell stimulatory factor 1, lymphocyte stimulatory factor 1
EliKine Mouse IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-4.
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: EliKine Mouse IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-4 in samples. An antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured