EliKine Mouse IL-4 ELISA Kit

Catalog Number: BYT-ORB3145556
Article Name: EliKine Mouse IL-4 ELISA Kit
Biozol Catalog Number: BYT-ORB3145556
Supplier Catalog Number: orb3145556
Alternative Catalog Number: BYT-ORB3145556-48, BYT-ORB3145556-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Mouse
Alternative Names: IL 4, IL4, BCGF-1, BCGF1, BSF1, IL-4, MGC79402, B cell growth factor 1, B_cell stimulatory factor 1, lymphocyte stimulatory factor 1
EliKine Mouse IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-4.
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: EliKine Mouse IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-4 in samples. An antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured