Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit

Artikelnummer: BYT-ORB3146879
Artikelname: Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit
Artikelnummer: BYT-ORB3146879
Hersteller Artikelnummer: orb3146879
Alternativnummer: BYT-ORB3146879-48, BYT-ORB3146879-5, BYT-ORB3146879-50, BYT-ORB3146879-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: PRKACA, MGC102831, MGC48865, PKACA, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha, cAMP-dependent protein kinase catalytic subunit alpha, isoform 1, protein kinase A catalytic subunit
This Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACA.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: This Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACA in samples. An antibody specific for PRKACA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACA bound in the initial step. The color development is stopped and the intensity of the color is measured