Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit

Catalog Number: BYT-ORB3146879
Article Name: Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit
Biozol Catalog Number: BYT-ORB3146879
Supplier Catalog Number: orb3146879
Alternative Catalog Number: BYT-ORB3146879-48, BYT-ORB3146879-5, BYT-ORB3146879-50, BYT-ORB3146879-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: PRKACA, MGC102831, MGC48865, PKACA, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha, cAMP-dependent protein kinase catalytic subunit alpha, isoform 1, protein kinase A catalytic subunit
This Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACA.
Range: Please inquire
Sensitivity: Please inquire
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: This Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACA in samples. An antibody specific for PRKACA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACA bound in the initial step. The color development is stopped and the intensity of the color is measured