BDNF Antibody (Biotin), Rabbit, Polyclonal

Produktübersicht

• Artikelname: BDNF Antibody (Biotin), Rabbit, Polyclonal
• Artikelnummer: BYT-ORB377477
• Hersteller Artikelnummer: orb377477
• Alternativnummer: BYT-ORB377477-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IF, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Synthetic peptide from the N-terminal of human BDNF
• Konjugation: Biotin
• Alternative Synonym: BDNF, BDNF_HUMAN, Brain-derived neurotrophic factor, Abrineurin, ANON2, MGC34632, Neuronal differentiation factor

Produktbeschreibung

Rabbit polyclonal antibody against BDNF conjugated to Biotin.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 27.9kDa
• NCBI: 001137277
• UniProt: P23560
• Puffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Target-Kategorie: BDNF

Anwendungsbeschreibung

• Application Verdünnung: WB (1:1000), ICC/IF (1:100)
• Anwendungsbeschreibung: Application Notes: A 1:1000 dilution of SPC-703 was sufficient for detection of BDNF on 293T Rapamycin-treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody

Bilder

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BDNF Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain, DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Secreted, Cytoplasm, Membrane-bound vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BDNF Antibody (D) Composite.

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.

Western blot analysis of Mouse Brain showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.