BDNF Antibody (Biotin), Rabbit, Polyclonal

Product Overview

• Article Name: BDNF Antibody (Biotin), Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB377477
• Supplier Catalog Number: orb377477
• Alternative Catalog Number: BYT-ORB377477-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, WB
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic peptide from the N-terminal of human BDNF
• Conjugation: Biotin
• Alternative Names: BDNF, BDNF_HUMAN, Brain-derived neurotrophic factor, Abrineurin, ANON2, MGC34632, Neuronal differentiation factor

Product Description

Rabbit polyclonal antibody against BDNF conjugated to Biotin.

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 27.9kDa
• NCBI: 001137277
• UniProt: P23560
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Target: BDNF

Application Notes

• Application Dilute: WB (1:1000), ICC/IF (1:100)
• Application Notes: Application Notes: A 1:1000 dilution of SPC-703 was sufficient for detection of BDNF on 293T Rapamycin-treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody

Images

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BDNF Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain, DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Secreted, Cytoplasm, Membrane-bound vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BDNF Antibody (D) Composite.

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.

Western blot analysis of Mouse Brain showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.