Phosphothreonine Antibody (Biotin), Rabbit, Polyclonal

Produktübersicht

• Artikelname: Phosphothreonine Antibody (Biotin), Rabbit, Polyclonal
• Artikelnummer: BYT-ORB396903
• Hersteller Artikelnummer: orb396903
• Alternativnummer: BYT-ORB396903-400
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IF, IP, WB
• Spezies Reaktivität: All
• Immunogen: Phosphothreonine conjugated to KLH
• Konjugation: Biotin
• Alternative Synonym: O-phospho-L-threonine, Phospho-threonine

Produktbeschreibung

Rabbit polyclonal antibody against Phosphothreonine conjugated to Biotin.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.25 mg/ml
• Puffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Target-Kategorie: Phosphothreonine

Anwendungsbeschreibung

• Application Verdünnung: WB (1:500), ICC/IF (1:60), ELISA (1:2000), IP (1:100)
• Anwendungsbeschreibung: Application Notes: 2 µg/ml of SPC-154 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium

Bilder

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite.

Western blot analysis of Mouse brain cell lysates showing detection of Phosphothreonine protein using Rabbit Anti-Phosphothreonine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:1000. Left: Treated with Vanadium, Right: Non-treated.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite.