Phosphothreonine Antibody (Biotin), Rabbit, Polyclonal

Product Overview

• Article Name: Phosphothreonine Antibody (Biotin), Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB396903
• Supplier Catalog Number: orb396903
• Alternative Catalog Number: BYT-ORB396903-400
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, IP, WB
• Species Reactivity: All
• Immunogen: Phosphothreonine conjugated to KLH
• Conjugation: Biotin
• Alternative Names: O-phospho-L-threonine, Phospho-threonine

Product Description

Rabbit polyclonal antibody against Phosphothreonine conjugated to Biotin.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.25 mg/ml
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Target: Phosphothreonine

Application Notes

• Application Dilute: WB (1:500), ICC/IF (1:60), ELISA (1:2000), IP (1:100)
• Application Notes: Application Notes: 2 µg/ml of SPC-154 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium

Images

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite.

Western blot analysis of Mouse brain cell lysates showing detection of Phosphothreonine protein using Rabbit Anti-Phosphothreonine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:1000. Left: Treated with Vanadium, Right: Non-treated.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite.