PARK7/DJ1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: PARK7/DJ1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: BYT-ORB402400
• Hersteller Artikelnummer: orb402400
• Alternativnummer: BYT-ORB402400-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC, WB
• Spezies Reaktivität: Mouse, Rat
• Immunogen: E. coli-derived rat PARK7 / DJ1 recombinant protein (Position: A2-D189).
• Konjugation: Unconjugated
• Alternative Synonym: Protein DJ-1, DJ-1, Contraception-associated protein 1

Produktbeschreibung

Anti-PARK7/DJ1 Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
• Molekulargewicht: 22 kDa
• UniProt: O88767
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: Western blot, 0.1-0.5µg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml ELISA (Cap), 1-5µg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Bilder

IHC analysis of PARK7 / DJ1 using anti-PARK7 / DJ1 antibody. PARK7 / DJ1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-PARK7 / DJ1 Antibody overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC ana

IHC analysis of PARK7 / DJ1 using anti-PARK7 / DJ1 antibody. PARK7 / DJ1 was detected in paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-PARK7 / DJ1 Antibody overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of PARK7 / DJ1 using anti-PARK7 / DJ1 antibody. PARK7 / DJ1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-PARK7 / DJ1 Antibody overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of PARK7 / DJ1 using anti-PARK7 / DJ1 antibody. PARK7 / DJ1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-PARK7 / DJ1 Antibody overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of PARK7 / DJ1 using anti-PARK7 / DJ1 antibody. PARK7 / DJ1 was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-PARK7 / DJ1 Antibody overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of PARK7 / DJ1 using anti-PARK7 / DJ1 antibody. PARK7 / DJ1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-PARK7 / DJ1 Antibody overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse liver ti