Human PARP1 protein, Unconjugated Preis auf Anfrage

Produktübersicht

• Artikelname: Human PARP1 protein, Unconjugated Preis auf Anfrage
• Artikelnummer: BYT-ORB420172
• Hersteller Artikelnummer: orb420172
• Alternativnummer: BYT-ORB420172-25
• Hersteller: Biorbyt
• Kategorie: Proteine/Peptide
• Applikation: SDS-PAGE, WB
• Konjugation: Unconjugated
• Alternative Synonym: Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, PPOL

Produktbeschreibung

Human PARP1 protein

Produkteigenschaften

• Konzentration: 1.0 mg/ml
• UniProt: P09874
• Puffer: 0.01% (w/v) Sodium Azide
• Reinheit: PARP1 (N-term ZF1) is an N-terminus His-Tag recombinant protein expressed in E.coli that corresponds to a fragment of the human PARP1 zinc finger domain. Analysis by SDS-PAGE and Coomassie staining resulted in ~13 kDa MW band and estimated purity 90%.
• Formulierung: Liquid (sterile filtered)

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: PARP1 (N-term ZF1) protein has been tested in SDS-Protein and western blot and is suitable as a control for immunological assays that use Anti-PARP1-ZF (RABBIT) Antibody (200-401-GM8). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to PARP-1 by western blotting

Bilder

SDS-PAGE results of PARP1 (N-term ZF1) Control Protein. Lane 1: reduced PARP1 N-Term ZF1 protein. Lane 2: Opal Prestained Molecular Weight Ladder. Lane 3: non-reduced PARP1 N-Term ZF1 protein. Load: 1 µg. 4-20% Lonza SDS-PAGE, Coomassie Stained, BioRad ChemiDoc Imaged.

Western Blot of recombinant PARP1 with rabbit anti-PARP1 (N-term ZF1) antibody. Lane 1: PARP1-Zinc Finger domain recombinant protein. Load: 0.05 µg per lane. Primary antibody: PARP1 (N-term ZF1) antibody at 1 µg/ml for overnight at 4C. Secondary antibody: HRP Gt-a-rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 overnight at 4C. Predicted/Observed size: 13 kDa for rPARP1 (N-term ZF1). Other band(s): none.