PARP1 (N-term ZF1) is an N-terminus His-Tag recombinant protein expressed in E.coli that corresponds to a fragment of the human PARP1 zinc finger domain. Analysis by SDS-PAGE and Coomassie staining resulted in ~13 kDa MW band and estimated purity 90%.
Form:
Liquid (sterile filtered)
Application Notes:
Application Notes: PARP1 (N-term ZF1) protein has been tested in SDS-Protein and western blot and is suitable as a control for immunological assays that use Anti-PARP1-ZF (RABBIT) Antibody (200-401-GM8). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to PARP-1 by western blotting
SDS-PAGE results of PARP1 (N-term ZF1) Control Protein. Lane 1: reduced PARP1 N-Term ZF1 protein. Lane 2: Opal Prestained Molecular Weight Ladder. Lane 3: non-reduced PARP1 N-Term ZF1 protein. Load: 1 µg. 4-20% Lonza SDS-PAGE, Coomassie Stained, BioRad ChemiDoc Imaged.
Western Blot of recombinant PARP1 with rabbit anti-PARP1 (N-term ZF1) antibody. Lane 1: PARP1-Zinc Finger domain recombinant protein. Load: 0.05 µg per lane. Primary antibody: PARP1 (N-term ZF1) antibody at 1 µg/ml for overnight at 4C. Secondary antibody: HRP Gt-a-rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 overnight at 4C. Predicted/Observed size: 13 kDa for rPARP1 (N-term ZF1). Other band(s): none.
* VAT and and shipping costs not included. Errors and price changes excepted
