Ribonuclease l (27 kD) is a completely nonspecific ribonuclease that hydrolyzes the phosphodiester bond of all four bases. It degrades any RNA to a mixture of mono-, di-, and trinucleotides and does not degrade DNA, although it does bind to DNA. It has a marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in RNase Protection Assays. It has a high specific activity which, coupled with its non-specificity, typically results in complete degradation of RNA using ng amounts of protein. Contains no endonuclease or exonuclease activity toward DNA substrates.
Molekulargewicht:
Theoretical MW: 27.0 kDa
Reinheit:
> 95% (SDS-PAGE)
Formulierung:
liquid (Supplied in 10 mM Tris-HCl pH 8.0, 200 mM NaCl and 50 % [v/v] glycerol)
Anwendungsbeschreibung:
degrades RNA to cyclic nucleotide monophosphates leaving a 5-OH and 2-, 3-cyclic monophosphate. Ribonuclease Protection Assays. Mapping or Quantitation of RNA by selective cleavage of single-strand regions. Eliminates RNA from DNA-and protein purification.
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