Ribonuclease l (27 kD) is a completely nonspecific ribonuclease that hydrolyzes the phosphodiester bond of all four bases. It degrades any RNA to a mixture of mono-, di-, and trinucleotides and does not degrade DNA, although it does bind to DNA. It has a
Clonality:
Recombinant
Molecular Weight:
27.0 kDa
Purity:
> 95 % (SDS-PAGE)
Form:
Liquid (Supplied in 10 mM Tris-HCl pH 8.0, 200 mM NaCl and 50 % [v/v] glycerol).
Application Notes:
Degrades RNA to cyclic nucleotide monophosphates leaving a 5-OH and 2-, 3-cyclic monophosphate Ribonuclease Protection Assays Mapping or Quantitation of RNA by selective cleavage of single-strand regions Eliminates RNA from DNA- and protein purification
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