136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
Target-Kategorie:
Alpha Synuclein
Application Verdünnung:
WB (1:1000)
Anwendungsbeschreibung:
Application Notes: A 1:1000 dilution of SPC-800 was sufficient for detection of Alpha Synuclein in 15 µg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody
Western blot analysis of Mouse Brain showing detection of ~14.46kDa Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~14.46kDa. Other Band (s): Higher molecular weight oligomers.
Western blot analysis of Human Recombinant Protein showing detection of Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: MW ladder. Lane 2: Alpha Synuclein. Lane 3: Beta Synuclein. Lane 4: Gamma Synuclein. Load: 0.5 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Alpha synuclein antibody shows cross-reactivity with beta synuclein but not gamma synuclein.
Western blot analysis of Human Truncated Alpha Synuclein Protein showing detection of Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: hASYN aa 1-114 (5 uL). Load: 5 uL. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.
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