Alpha Synuclein Antibody (Biotin), Rabbit, Polyclonal

Product Overview

• Article Name: Alpha Synuclein Antibody (Biotin), Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB536155
• Supplier Catalog Number: orb536155
• Alternative Catalog Number: BYT-ORB536155-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant Human alpha Synuclein Fibril
• Conjugation: Biotin
• Alternative Names: Alpha Synuclein, alpha-Synuclein, SNCA, Snca, SYN, alphaSYN, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease familial 1, PARK1, PARK 1, PARK4, PARK 4, Parkinson disease (autosomal dominant, Lewy body) 4

Product Description

Rabbit polyclonal antibody against alpha Synuclein conjugated to Biotin

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 14.46kDa
• NCBI: 000336
• UniProt: P37840
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Target: Alpha Synuclein

Application Notes

• Application Dilute: WB (1:1000)
• Application Notes: Application Notes: A 1:1000 dilution of SPC-800 was sufficient for detection of Alpha Synuclein in 15 µg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody

Images

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Tissue: Cerebellum. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody.

Western blot analysis of Mouse Brain showing detection of ~14.46kDa Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~14.46kDa. Other Band (s): Higher molecular weight oligomers.

Western blot analysis of Human Recombinant Protein showing detection of Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: MW ladder. Lane 2: Alpha Synuclein. Lane 3: Beta Synuclein. Lane 4: Gamma Synuclein. Load: 0.5 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Alpha synuclein antibody shows cross-reactivity with beta synuclein but not gamma synuclein.

Western blot analysis of Human Truncated Alpha Synuclein Protein showing detection of Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: hASYN aa 1-114 (5 uL). Load: 5 uL. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.