HMGB1 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: HMGB1 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB704161
• Hersteller Artikelnummer: orb704161
• Alternativnummer: BYT-ORB704161-100,BYT-ORB704161-200,BYT-ORB704161-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human HMGB1 (91-190/215aa)
• Konjugation: Unconjugated
• Alternative Synonym: High mobility group protein B1, Amphoterin, High mobility group 1, High Mobility Group Box 1, High mobility group protein 1, HMG3, HMGB 1, HMGB-1, Hmgb1 protein, Nonhistone chromosomal protein HMG1, SBP 1, SBP-1, Sulfoglucuronyl carbohydrate binding protein, HMGB1_HUMAN.

Produktbeschreibung

HMGB1 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 25 kDa
• UniProt: P09429
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: HMGB1

Anwendungsbeschreibung

• Application Verdünnung: IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P analysis of human gastric carcinoma using HMGB1 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of mouse kidney using HMGB1 Polyclonal Antibody at 1:200 dilution.