HMGB1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: HMGB1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB704161
• Supplier Catalog Number: orb704161
• Alternative Catalog Number: BYT-ORB704161-100,BYT-ORB704161-200,BYT-ORB704161-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human HMGB1 (91-190/215aa)
• Conjugation: Unconjugated
• Alternative Names: High mobility group protein B1, Amphoterin, High mobility group 1, High Mobility Group Box 1, High mobility group protein 1, HMG3, HMGB 1, HMGB-1, Hmgb1 protein, Nonhistone chromosomal protein HMG1, SBP 1, SBP-1, Sulfoglucuronyl carbohydrate binding protein, HMGB1_HUMAN.

Product Description

HMGB1 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 25 kDa
• UniProt: P09429
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: HMGB1

Application Notes

• Application Dilute: IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (orb704161) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P analysis of human gastric carcinoma using HMGB1 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of mouse kidney using HMGB1 Polyclonal Antibody at 1:200 dilution.