Human Carbohydrate Antigen 19-5 (CA19-5) ELISA Kit

Artikelnummer: BYT-ORB780925
Artikelname: Human Carbohydrate Antigen 19-5 (CA19-5) ELISA Kit
Artikelnummer: BYT-ORB780925
Hersteller Artikelnummer: orb780925
Alternativnummer: BYT-ORB780925-48, BYT-ORB780925-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: CA19-5
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Carbohydrate Antigen 19-5(CA19-5) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carbohydrate Antigen 19-5(CA19-5).
Konzentration: 200 ng/mL
Detektionsbereich: 3.13-200 ng/mL
Sensitivitaet: 0.85 ng/mL
Proben: Serum, plasma and other biological fluids
Anwendungsbeschreibung: Application Notes: standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CA19-5 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA19-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CA19-5 in the samples is then determined by comparing the OD of the samples to the standard curve