Human Carbohydrate Antigen 19-5 (CA19-5) ELISA Kit
Biozol Catalog Number:
BYT-ORB780925
Supplier Catalog Number:
orb780925
Alternative Catalog Number:
BYT-ORB780925-48, BYT-ORB780925-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Human
Alternative Names:
CA19-5
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Carbohydrate Antigen 19-5(CA19-5) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carbohydrate Antigen 19-5(CA19-5).
Concentration:
200 ng/mL
Range:
3.13-200 ng/mL
Sensitivity:
0.85 ng/mL
Samples:
Serum, plasma and other biological fluids
Application Notes:
Application Notes: standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CA19-5 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA19-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CA19-5 in the samples is then determined by comparing the OD of the samples to the standard curve
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