General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit

Produktübersicht

• Artikelname: General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit
• Artikelnummer: BYT-ORB781881
• Hersteller Artikelnummer: orb781881
• Alternativnummer: BYT-ORB781881-48, BYT-ORB781881-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: All
• Alternative Synonym: 8-EpiPGF2-alpha ELISA Kit, 8epi-PGF2a ELISA Kit

Produktbeschreibung

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a).

Produkteigenschaften

• Konzentration: 2000 pg/mL
• Detektionsbereich: 31.25-2000 pg/mL
• Sensitivitaet: 9.23 pg/mL
• Proben: serum, plasma and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-epi-PGF2a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-epi-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of 8-epi-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve