General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit
Biozol Catalog Number:
BYT-ORB781881
Supplier Catalog Number:
orb781881
Alternative Catalog Number:
BYT-ORB781881-48, BYT-ORB781881-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
All
Alternative Names:
8-EpiPGF2-alpha ELISA Kit, 8epi-PGF2a ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a).
Concentration:
2000 pg/mL
Range:
31.25-2000 pg/mL
Sensitivity:
9.23 pg/mL
Samples:
serum, plasma and other biological fluids
Application Notes:
Application Notes: standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-epi-PGF2a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-epi-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of 8-epi-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve
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