General 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit
Artikelnummer:
BYT-ORB781992
Hersteller Artikelnummer:
orb781992
Alternativnummer:
BYT-ORB781992-48, BYT-ORB781992-96
Hersteller:
Biorbyt
Kategorie:
Kits/Assays
Applikation:
ELISA
Spezies Reaktivität:
All
Alternative Synonym:
12HETE ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-Hydroxyeicosatetraenoic Acid(12-HETE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-Hydroxyeicosatetraenoic Acid(12-HETE).
Konzentration:
1000 ng/mL
Detektionsbereich:
15.63-1000 ng/mL
Sensitivitaet:
5.13 ng/mL
Proben:
serum, plasma and other biological fluids
Anwendungsbeschreibung:
Application Notes: standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-HETE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
