General 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit
Biozol Catalog Number:
BYT-ORB781992
Supplier Catalog Number:
orb781992
Alternative Catalog Number:
BYT-ORB781992-48, BYT-ORB781992-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
All
Alternative Names:
12HETE ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-Hydroxyeicosatetraenoic Acid(12-HETE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-Hydroxyeicosatetraenoic Acid(12-HETE).
Concentration:
1000 ng/mL
Range:
15.63-1000 ng/mL
Sensitivity:
5.13 ng/mL
Samples:
serum, plasma and other biological fluids
Application Notes:
Application Notes: standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-HETE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve
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