Dog Pro Atrial Natriuretic Peptide (Pro-ANP) ELISA Kit

Artikelnummer: BYT-ORB782526
Artikelname: Dog Pro Atrial Natriuretic Peptide (Pro-ANP) ELISA Kit
Artikelnummer: BYT-ORB782526
Hersteller Artikelnummer: orb782526
Alternativnummer: BYT-ORB782526-48, BYT-ORB782526-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Canine
Alternative Synonym: NT-proANP ELISA Kit, Pro Atrial Natriuretic Peptide ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pro Atrial Natriuretic Peptide(Pro-ANP).
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.053 ng/mL
UniProt: P07499
Proben: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Dog Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve