Dog Pro Atrial Natriuretic Peptide (Pro-ANP) ELISA Kit
Biozol Catalog Number:
BYT-ORB782526
Supplier Catalog Number:
orb782526
Alternative Catalog Number:
BYT-ORB782526-48, BYT-ORB782526-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Canine
Alternative Names:
NT-proANP ELISA Kit, Pro Atrial Natriuretic Peptide ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pro Atrial Natriuretic Peptide(Pro-ANP).
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Dog Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve
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