Avi Tag Monoclonal Antibody, IgG2a, Clone: [9G3B5], Unconjugated, Mouse

Produktübersicht

• Artikelname: Avi Tag Monoclonal Antibody, IgG2a, Clone: [9G3B5], Unconjugated, Mouse
• Artikelnummer: CSB-MA000101M0M
• Hersteller Artikelnummer: CSB-MA000101M0m
• Alternativnummer: CSB-MA000101M0M-100UL, CSB-MA000101M0M-50UL
• Hersteller: Cusabio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, IP, WB
• Spezies Reaktivität: All
• Konjugation: Unconjugated
• Alternative Synonym: Avi Tag

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [9G3B5]
• Isotyp: IgG2a
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: Avi Tag

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB: 1:1000-1:640000, IP: 2µl-5µl

Bilder

Immunoprecipitating Avi tag in 293F whole cell lysate transfected with avi tag
Lane 1: Mouse control IgG instead of CSB-MA000101M0m in 293F whole cell lysate transfected with avi tag.Lane 2: CSB-MA000101M0m (1µg) + 293F whole cell lysate transfected with avi tag (500µg)
Lane 3: 293F whole cell lysate transfected with avi tag(40µg)
For western blotting, the blot was detected with CSB-MA000101M0m at 1:1000, and a HRP-conjugated Goat anti Mouse antibody was used as the secondary antibody at 1:50000

Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli and Mammalian cellAll lanes: Avi tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40, 35, 30, 70, 40, 33, 35, 40 KDa
Observed band size: 40, 35, 30, 70, 40, 33, 35, 4 KDa
Exposure time:1min

Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coliAll lanes: Avi tag antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 81.2, 67, 66.9, 66.9 KDa
Observed band size: 82, 67, 67, 67KDa
Exposure time:5min

Western Blot
Positive WB detected in: Recombinant protein from Mammalian cell with Avi tag from E.coli at 100ng, 50ng, 25ng, 12.5ng, 6.25ng, 3.125ngAll lanes: Avi tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40 KDa
Observed band size: 40 KDa
Exposure time:1min

Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from Mammalian cell Avi tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000, 1:12800000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40 KDa
Observed band size: 40 KDa
Exposure time:5min