Avi Tag Monoclonal Antibody, IgG2a, Clone: [9G3B5], Unconjugated, Mouse

Product Overview

• Article Name: Avi Tag Monoclonal Antibody, IgG2a, Clone: [9G3B5], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA000101M0M
• Supplier Catalog Number: CSB-MA000101M0m
• Alternative Catalog Number: CSB-MA000101M0M-100UL, CSB-MA000101M0M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IP, WB
• Species Reactivity: All
• Conjugation: Unconjugated
• Alternative Names: Avi Tag

Product Properties

• Clonality: Monoclonal
• Clone Designation: [9G3B5]
• Isotype: IgG2a
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: Avi Tag

Application Notes

• Application Dilute: Recommended dilution: WB: 1:1000-1:640000, IP: 2µl-5µl

Images

Immunoprecipitating Avi tag in 293F whole cell lysate transfected with avi tag
Lane 1: Mouse control IgG instead of CSB-MA000101M0m in 293F whole cell lysate transfected with avi tag.Lane 2: CSB-MA000101M0m (1µg) + 293F whole cell lysate transfected with avi tag (500µg)
Lane 3: 293F whole cell lysate transfected with avi tag(40µg)
For western blotting, the blot was detected with CSB-MA000101M0m at 1:1000, and a HRP-conjugated Goat anti Mouse antibody was used as the secondary antibody at 1:50000

Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli and Mammalian cellAll lanes: Avi tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40, 35, 30, 70, 40, 33, 35, 40 KDa
Observed band size: 40, 35, 30, 70, 40, 33, 35, 4 KDa
Exposure time:1min

Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coliAll lanes: Avi tag antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 81.2, 67, 66.9, 66.9 KDa
Observed band size: 82, 67, 67, 67KDa
Exposure time:5min

Western Blot
Positive WB detected in: Recombinant protein from Mammalian cell with Avi tag from E.coli at 100ng, 50ng, 25ng, 12.5ng, 6.25ng, 3.125ngAll lanes: Avi tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40 KDa
Observed band size: 40 KDa
Exposure time:1min

Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from Mammalian cell Avi tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000, 1:12800000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40 KDa
Observed band size: 40 KDa
Exposure time:5min