CANX Monoclonal Antibody, IgG2b, Clone: [15B1A7], Unconjugated, Mouse

Produktübersicht

• Artikelname: CANX Monoclonal Antibody, IgG2b, Clone: [15B1A7], Unconjugated, Mouse
• Artikelnummer: CSB-MA004485A1M
• Hersteller Artikelnummer: CSB-MA004485A1m
• Alternativnummer: CSB-MA004485A1M-100UL, CSB-MA004485A1M-50UL
• Hersteller: Cusabio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, p90 antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [15B1A7]
• Isotyp: IgG2b
• UniProt: P27824
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: CANX

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, FC:1:50-1:200

Bilder

Overlay Peak curve showing Hela cells stained with CSB-MA004485A1m (red line) at 1:140. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

CSB-MA004485A1m

Immunofluorescence staining of Hela cells with CANX CSB-MA004485A1m at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Immunofluorescence staining of HepG2 cells with CANX CSB-MA004485A1m at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Western Blot
Positive WB detected in: MCF7 whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate
All lanes: CANX antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 68, 72, 56 kDa
Observed band size: 90 KDa
Exposure time:1min