CANX Monoclonal Antibody, IgG2b, Clone: [15B1A7], Unconjugated, Mouse

Product Overview

• Article Name: CANX Monoclonal Antibody, IgG2b, Clone: [15B1A7], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA004485A1M
• Supplier Catalog Number: CSB-MA004485A1m
• Alternative Catalog Number: CSB-MA004485A1M-100UL, CSB-MA004485A1M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, p90 antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [15B1A7]
• Isotype: IgG2b
• UniProt: P27824
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: CANX

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, FC:1:50-1:200

Images

Overlay Peak curve showing Hela cells stained with CSB-MA004485A1m (red line) at 1:140. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

CSB-MA004485A1m

Immunofluorescence staining of Hela cells with CANX CSB-MA004485A1m at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Immunofluorescence staining of HepG2 cells with CANX CSB-MA004485A1m at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Western Blot
Positive WB detected in: MCF7 whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate
All lanes: CANX antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 68, 72, 56 kDa
Observed band size: 90 KDa
Exposure time:1min