MB Monoclonal Antibody, IgG1, Clone: [7A3D2], Unconjugated, Mouse

Produktübersicht

• Artikelname: MB Monoclonal Antibody, IgG1, Clone: [7A3D2], Unconjugated, Mouse
• Artikelnummer: CSB-MA079101A0M
• Hersteller Artikelnummer: CSB-MA079101A0m
• Alternativnummer: CSB-MA079101A0M-100UG, CSB-MA079101A0M-50UG
• Hersteller: Cusabio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: Myoglobin, MB, MGC13548, PVALB

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [7A3D2]
• Isotyp: IgG1
• UniProt: P02144
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: MB

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200

Bilder

Immunofluorescent analysis of MCF-7 cells using CSB-MA079101A0m at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

Immunohistochemical of paraffin-embedded human heart tissue using CSB-MA079101A0m at dilution of 1:100

Western Blot
All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 0.3µg/ml
Lane 1: Mouse heart muscle lysate
Lane 2: Recombinant Myoglobin at 10µg
Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa
Additional bands at: 120 kDa

Western Blot
All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 1µg/ml
Lane 1: Myoglobin transfected 293 cell lysate
Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa
Additional bands at: 23, 25, 40 kDa

Western Blot
Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue
All lanes: MB antibody at 1:2000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa