MB Monoclonal Antibody, IgG1, Clone: [7A3D2], Unconjugated, Mouse

Product Overview

• Article Name: MB Monoclonal Antibody, IgG1, Clone: [7A3D2], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA079101A0M
• Supplier Catalog Number: CSB-MA079101A0m
• Alternative Catalog Number: CSB-MA079101A0M-100UG, CSB-MA079101A0M-50UG
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: Myoglobin, MB, MGC13548, PVALB

Product Properties

• Clonality: Monoclonal
• Clone Designation: [7A3D2]
• Isotype: IgG1
• UniProt: P02144
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: MB

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200

Images

Immunofluorescent analysis of MCF-7 cells using CSB-MA079101A0m at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

Immunohistochemical of paraffin-embedded human heart tissue using CSB-MA079101A0m at dilution of 1:100

Western Blot
All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 0.3µg/ml
Lane 1: Mouse heart muscle lysate
Lane 2: Recombinant Myoglobin at 10µg
Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa
Additional bands at: 120 kDa

Western Blot
All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 1µg/ml
Lane 1: Myoglobin transfected 293 cell lysate
Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa
Additional bands at: 23, 25, 40 kDa

Western Blot
Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue
All lanes: MB antibody at 1:2000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa