BTN3A1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: BTN3A1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA002873LA01HU
• Hersteller Artikelnummer: CSB-PA002873LA01HU
• Alternativnummer: CSB-PA002873LA01HU-100UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: BT3A1_HUMAN antibody, BTN3A1 antibody, Butyrophilin subfamily 3 member A1 antibody, CD277 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: O00481
• Puffer: Preservative: 0.02% sodium azide<br />Constituents: PBS containing 50% glycerol pH 7.3
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: BTN3A1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:1000,IF:1:20-1:200

Bilder

Immunohistochemistry of paraffin-embedded human colon c

Immunofluorescence staining of Hela cell with CSB-PA002873LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ImmunofluorescencestainingofHelacellwith5%goatserum,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehydeandblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasAlexaFluor488-congugatedAffiniPureGoatAnti-RabbitIgG(H+L).

Immunofluorescence staining of SH-SY5Y cell with CSB-PA002873LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of SH-SY5Y cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: U937 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate
All lanes: BTN3A1 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40-58 kDa
Observed band size: 48 kDa