BTN3A1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: BTN3A1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA002873LA01HU
• Supplier Catalog Number: CSB-PA002873LA01HU
• Alternative Catalog Number: CSB-PA002873LA01HU-100UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: BT3A1_HUMAN antibody, BTN3A1 antibody, Butyrophilin subfamily 3 member A1 antibody, CD277 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: O00481
• Buffer: Preservative: 0.02% sodium azide<br />Constituents: PBS containing 50% glycerol pH 7.3
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: BTN3A1

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:1000,IF:1:20-1:200

Images

Immunohistochemistry of paraffin-embedded human colon c

Immunofluorescence staining of Hela cell with CSB-PA002873LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ImmunofluorescencestainingofHelacellwith5%goatserum,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehydeandblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasAlexaFluor488-congugatedAffiniPureGoatAnti-RabbitIgG(H+L).

Immunofluorescence staining of SH-SY5Y cell with CSB-PA002873LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of SH-SY5Y cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: U937 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate
All lanes: BTN3A1 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40-58 kDa
Observed band size: 48 kDa