CCND3 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CCND3 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA004814ESR2HU
• Hersteller Artikelnummer: CSB-PA004814ESR2HU
• Alternativnummer: CSB-PA004814ESR2HU-100UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: CCND 3 antibody, Ccnd3 antibody, CCND3_HUMAN antibody, CyclinD3 antibody, D3 type cyclin antibody, G1 S specific cyclin D3 antibody, G1/S specific cyclin D3 antibody, G1/S-specific cyclin-D3 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: P30281
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: CCND3

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:5000-1:2000, IF:1:20-1:100

Bilder

Western Blot
Positive WB detected

Immunofluorescence staining of Hela cell with CSB-PA004814ESR2HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with CSB-PA004814ESR2HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: K562 whole cell lysate(20µg), JK whole cell lysate(20µg), Raji whole cell lysate(20µg), Hela whole cell lysate(20µg), A549 whole cell lysate(20µg)
All lanes:CCND3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 33,23,25,10 kDa
Observed band size: 33 kDa
Exposure time: 120s