CCND3 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA004814ESR2HU
- Images (6)
| Article Name: | CCND3 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA004814ESR2HU |
| Supplier Catalog Number: | CSB-PA004814ESR2HU |
| Alternative Catalog Number: | CSB-PA004814ESR2HU-100UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | CCND 3 antibody, Ccnd3 antibody, CCND3_HUMAN antibody, CyclinD3 antibody, D3 type cyclin antibody, G1 S specific cyclin D3 antibody, G1/S specific cyclin D3 antibody, G1/S-specific cyclin-D3 antibody |
| Clonality: | Polyclonal |
| UniProt: | P30281 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | CCND3 |
| Application Dilute: | Recommended dilution: WB:1:5000-1:2000, IF:1:20-1:100 |
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Western Blot Positive WB detected |
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Immunofluorescence staining of Hela cell with CSB-PA004814ESR2HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunofluorescence staining of A549 cell with CSB-PA004814ESR2HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Western Blot Positive WB detected in: K562 whole cell lysate(20µg), JK whole cell lysate(20µg), Raji whole cell lysate(20µg), Hela whole cell lysate(20µg), A549 whole cell lysate(20µg) All lanes:CCND3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 33,23,25,10 kDa Observed band size: 33 kDa Exposure time: 120s |
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