CIDEA Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CIDEA Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA005431LA01HU
• Hersteller Artikelnummer: CSB-PA005431LA01HU
• Alternativnummer: CSB-PA005431LA01HU-100UG, CSB-PA005431LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: CIDEACell death activator CIDE-A antibody, Cell death-inducing DFFA-like effector A antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: O60543
• Puffer: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
• Reinheit: Affinity-chromatography purified
• Formulierung: Liquid
• Target-Kategorie: CIDEA

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IF:1:20-1:200

Bilder

Im

Immunofluorescent analysis of Hela cells using CSB-PA005431LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescence staining of Hela cell with CSB-PA005431LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with CSB-PA005431LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate, JK whole cell lysate, Mouse Liver tissue lysate
All lanes: CIDEA antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 55 kDa