CIDEA Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CIDEA Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA005431LA01HU
• Supplier Catalog Number: CSB-PA005431LA01HU
• Alternative Catalog Number: CSB-PA005431LA01HU-100UG, CSB-PA005431LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: CIDEACell death activator CIDE-A antibody, Cell death-inducing DFFA-like effector A antibody

Product Properties

• Clonality: Polyclonal
• UniProt: O60543
• Buffer: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
• Purity: Affinity-chromatography purified
• Form: Liquid
• Target: CIDEA

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:2000, IF:1:20-1:200

Images

Im

Immunofluorescent analysis of Hela cells using CSB-PA005431LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescence staining of Hela cell with CSB-PA005431LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with CSB-PA005431LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate, JK whole cell lysate, Mouse Liver tissue lysate
All lanes: CIDEA antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 55 kDa