DVL2 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: DVL2 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA007286ESR1HU
• Hersteller Artikelnummer: CSB-PA007286ESR1HU
• Alternativnummer: CSB-PA007286ESR1HU-100UL, CSB-PA007286ESR1HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Dishevelled 2 (homologous to Drosophila dsh) antibody, Dishevelled dsh homolog 2 antibody, dishevelled segment polarity protein 2 antibody, Dishevelled-2 antibody, Dishevelled2 antibody, DSH homolog 2 antibody, DVL 2 antibody, Dvl2 antibody, DVL2_HUMAN antibody, Segment polarity protein dishevelled homolog DVL 2 antibody, Segment polarity protein dishevelled homolog DVL-2 antibody, Segment polarity protein dishevelled homolog DVL2 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: O14641
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: DVL2

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:500, IF:1:50-1:200

Bilder

Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA007286ESR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA007286ESR1HU at dilution of 1:100

Immunofluorescence staining of A549 cells with CSB-PA007286ESR1HU at 1:75, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

IHC image of CSB-PA007286ESR1HU diluted at 1:227 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA007286ESR1HU diluted at 1:227 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: DVL2 antibody at 2.3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa