PPP2CB Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA00985A0RB
Artikelname: PPP2CB Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA00985A0RB
Hersteller Artikelnummer: CSB-PA00985A0Rb
Alternativnummer: CSB-PA00985A0RB-100UG, CSB-PA00985A0RB-50UG
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: PP2A beta antibody, PP2A-beta antibody, PP2AB_HUMAN antibody, PP2Abeta antibody, PP2CB antibody, Ppp2cb antibody, Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody, Protein phosphatase 2 catalytic subunit beta isozyme antibody, Protein phosphatase 2, catalytic subunit, beta isoform antibody, Protein phosphatase 2A catalytic subunit beta isoform antibody, Protein phosphatase type 2A catalytic subunit antibody, Serine/threonine protein phosphatase 2A catalytic subunit beta antibody, Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
Klonalität: Polyclonal
UniProt: P62714
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Reinheit: >95%, Protein G purified
Formulierung: Liquid
Target-Kategorie: PPP2CB
Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:100-1:500, IF:1:50-1:200
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00985A0Rb at dilution of 1:50
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA00985A0Rb at dilution of 1:100
Immunofluorescent analysis of Hela cells using CSB-PA00985A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IHC image of CSB-PA00985A0Rb diluted at 1:260 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA00985A0Rb diluted at 1:260 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot
All lanes: Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2µg/ml + EC109 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa