Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00985A0Rb at dilution of 1:50
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA00985A0Rb at dilution of 1:100
Immunofluorescent analysis of Hela cells using CSB-PA00985A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IHC image of CSB-PA00985A0Rb diluted at 1:260 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA00985A0Rb diluted at 1:260 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot All lanes: Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2µg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
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