RHD Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: RHD Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA019677LA01HU
• Hersteller Artikelnummer: CSB-PA019677LA01HU
• Alternativnummer: CSB-PA019677LA01HU-100UG, CSB-PA019677LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Blood group Rh(D) polypeptide antibody, Blood group--Rhesus system D polypeptide antibody, CD240D antibody, D antigen (DCS) antibody, DIIIc antibody, MGC165007 antibody, RH antibody, Rh blood group antigen Evans antibody, Rh blood group D antigen antibody, Rh polypeptide 2 antibody, RH30 antibody, Rh4 antibody, RHCED antibody, Rhd antibody, RHD_HUMAN antibody, RhDCw antibody, RHDel antibody, RHDVA(TT) antibody, Rhesus blood group D antigen allele DIII type 7 antibody, Rhesus D antigen antibody, Rhesus system D polypeptide antibody, RhII antibody, RhK562-II antibody, RhPI antibody, RHPII antibody, RHXIII antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q02161
• Puffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: RHD

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:3000, IF:1:20-1:100

Bilder

Immunofluorescence staining of Hela cell with CSB-PA019677LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: K562 whole cell lysate(20µg), Hela whole cell lysate(20µg), HepG2 whole cell lysate(20µg), LO2 whole cell lysate(20µg)
All lanes: RHD antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 46,35,54,44,42 kDa
Observed band size: 35 kDa
Exposure time: 60s