RHD Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: RHD Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA019677LA01HU
• Supplier Catalog Number: CSB-PA019677LA01HU
• Alternative Catalog Number: CSB-PA019677LA01HU-100UG, CSB-PA019677LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: Blood group Rh(D) polypeptide antibody, Blood group--Rhesus system D polypeptide antibody, CD240D antibody, D antigen (DCS) antibody, DIIIc antibody, MGC165007 antibody, RH antibody, Rh blood group antigen Evans antibody, Rh blood group D antigen antibody, Rh polypeptide 2 antibody, RH30 antibody, Rh4 antibody, RHCED antibody, Rhd antibody, RHD_HUMAN antibody, RhDCw antibody, RHDel antibody, RHDVA(TT) antibody, Rhesus blood group D antigen allele DIII type 7 antibody, Rhesus D antigen antibody, Rhesus system D polypeptide antibody, RhII antibody, RhK562-II antibody, RhPI antibody, RHPII antibody, RHXIII antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q02161
• Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: RHD

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:3000, IF:1:20-1:100

Images

Immunofluorescence staining of Hela cell with CSB-PA019677LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: K562 whole cell lysate(20µg), Hela whole cell lysate(20µg), HepG2 whole cell lysate(20µg), LO2 whole cell lysate(20µg)
All lanes: RHD antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 46,35,54,44,42 kDa
Observed band size: 35 kDa
Exposure time: 60s