SIRPB1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SIRPB1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA021335LA01HU
• Hersteller Artikelnummer: CSB-PA021335LA01HU
• Alternativnummer: CSB-PA021335LA01HU-100UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: SIRPB1 antibody, Signal-regulatory protein beta-1 antibody, SIRP-beta-1 antibody, CD172 antigen-like family member B antibody, CD antigen CD172b antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: O00241
• Puffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: SIRPB1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:500-2000, IF:1:10-1:100

Bilder

Immunofluorescent analysis of Hela cel

Immunofluorescence staining of U251 cell with CSB-PA021335LA01HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with CSB-PA021335LA01HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: THP-1 whole cell lysate(20µg), U87 whole cell lysate(20µg), A549 whole cell lysate(20µg), PC-3 whole cell lysate(20µg)
All lanes: SIRPB1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43,19 kDa
Observed band size: 43,86 kDa
Exposure time: 120s