SIRPB1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SIRPB1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA021335LA01HU
• Supplier Catalog Number: CSB-PA021335LA01HU
• Alternative Catalog Number: CSB-PA021335LA01HU-100UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: SIRPB1 antibody, Signal-regulatory protein beta-1 antibody, SIRP-beta-1 antibody, CD172 antigen-like family member B antibody, CD antigen CD172b antibody

Product Properties

• Clonality: Polyclonal
• UniProt: O00241
• Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: SIRPB1

Application Notes

• Application Dilute: Recommended dilution: WB:500-2000, IF:1:10-1:100

Images

Immunofluorescent analysis of Hela cel

Immunofluorescence staining of U251 cell with CSB-PA021335LA01HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with CSB-PA021335LA01HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: THP-1 whole cell lysate(20µg), U87 whole cell lysate(20µg), A549 whole cell lysate(20µg), PC-3 whole cell lysate(20µg)
All lanes: SIRPB1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43,19 kDa
Observed band size: 43,86 kDa
Exposure time: 120s