YWHAH Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: YWHAH Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA02174A0RB
• Hersteller Artikelnummer: CSB-PA02174A0Rb
• Alternativnummer: CSB-PA02174A0RB-100UG, CSB-PA02174A0RB-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: 14 3 3 protein eta antibody, 14-3-3 eta antibody, 14-3-3 protein eta antibody, 1433F_HUMAN antibody, Brain protein 14-3-3, eta isoform antibody, HGNC:12853 antibody, Protein AS1 antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta antibody, Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody, YWHA 1 antibody, YWHA1 antibody, Ywhah antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q04917
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: YWHAH

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:500, IF:1:50-1:200

Bilder

Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA02174A0Rb at dilution of 1:20

Western blot
All lanes: YWHAH antibody at 2µg/ml + Mouse spleen tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa

Western Blot
Positive WB detected in: HepG2 whole cell lysate, Mouse kidney tissue, Mouse brain tissue, Mouse thymus tissue
All lanes: YWHAH antibody at 4.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa

Immunofluorescence staining of Hela cells with CSB-PA02174A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.