YWHAH Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA02174A0RB
- Images (6)
| Article Name: | YWHAH Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA02174A0RB |
| Supplier Catalog Number: | CSB-PA02174A0Rb |
| Alternative Catalog Number: | CSB-PA02174A0RB-100UG, CSB-PA02174A0RB-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Human, Mouse |
| Conjugation: | Unconjugated |
| Alternative Names: | 14 3 3 protein eta antibody, 14-3-3 eta antibody, 14-3-3 protein eta antibody, 1433F_HUMAN antibody, Brain protein 14-3-3, eta isoform antibody, HGNC:12853 antibody, Protein AS1 antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta antibody, Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody, YWHA 1 antibody, YWHA1 antibody, Ywhah antibody |
| Clonality: | Polyclonal |
| UniProt: | Q04917 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | YWHAH |
| Application Dilute: | Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:500, IF:1:50-1:200 |
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Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA02174A0Rb at dilution of 1:20 |
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Western blot All lanes: YWHAH antibody at 2µg/ml + Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa |
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Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse kidney tissue, Mouse brain tissue, Mouse thymus tissue All lanes: YWHAH antibody at 4.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa |
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Immunofluorescence staining of Hela cells with CSB-PA02174A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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