PTDSS1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA04305A0RB
- Bilder (5)
| Artikelname: | PTDSS1 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA04305A0RB |
| Hersteller Artikelnummer: | CSB-PA04305A0Rb |
| Alternativnummer: | CSB-PA04305A0RB-100UL, CSB-PA04305A0RB-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PTDSS1 antibody, KIAA0024 antibody, PSSA antibody, Phosphatidylserine synthase 1 antibody, PSS-1 antibody, PtdSer synthase 1 antibody, EC 2.7.8.29 antibody, Serine-exchange enzyme I antibody |
| Klonalität: | Polyclonal |
| UniProt: | P48651 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | PTDSS1 |
| Application Verdünnung: | Recommended dilution: WB:1:1000-1:3000 , IHC:1:100-1:300 |
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IHC image of CSB-PA04305A0Rb diluted at 1:150 and staining in paraffin-embedded humanpancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody |
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IHC image of CSB-PA04305A0Rb diluted at 1:150 and staining in paraffin-embedded humanpancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody |
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Western Blot Positive WB detected in: 293T whole cell lysate(20µg), K562 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), MCF7 whole cell lysate(20µg All lanes: PTDSS1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 56,39,35 kDa Observed band size: 35 kDa Exposure time: 60s |
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