PTDSS1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA04305A0RB
Article Name: PTDSS1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA04305A0RB
Supplier Catalog Number: CSB-PA04305A0Rb
Alternative Catalog Number: CSB-PA04305A0RB-100UL, CSB-PA04305A0RB-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: PTDSS1 antibody, KIAA0024 antibody, PSSA antibody, Phosphatidylserine synthase 1 antibody, PSS-1 antibody, PtdSer synthase 1 antibody, EC 2.7.8.29 antibody, Serine-exchange enzyme I antibody
Clonality: Polyclonal
UniProt: P48651
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: PTDSS1
Application Dilute: Recommended dilution: WB:1:1000-1:3000 , IHC:1:100-1:300
IHC image of CSB-PA04305A0Rb diluted at 1:150 and staining in paraffin-embedded humanpancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA04305A0Rb diluted at 1:150 and staining in paraffin-embedded humanpancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in: 293T whole cell lysate(20µg), K562 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), MCF7 whole cell lysate(20µg
All lanes: PTDSS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 56,39,35 kDa
Observed band size: 35 kDa
Exposure time: 60s